Alveolar Macrophages Isolated Directly From Human Cytomegalovirus (HCMV)-Seropositive Individuals Are Sites of HCMV Reactivation In Vivo. Poole E, Juss JK, Krishna B, Herre J, Chilvers ER, Sinclair J. J Infect Dis. 2014 Dec 30. pii: jiu837. [Epub ahead of print]

Glucocorticosteroids trigger reactivation of human cytomegalovirus from latently infected myeloid cells and increase the risk for HCMV infection in D+R+ liver transplant patients. Van Damme E, Sauviller S, Lau B, Kesteleyn B, Griffiths P, Burroughs A, Emery V, Sinclair J, Van Loock M. J Gen Virol. 2015 Jan;96(Pt 1):131-43. doi: 10.1099/vir.0.069872-0. Epub 2014 Oct 13.

Latency-associated viral interleukin-10 (IL-10) encoded by human cytomegalovirus modulates cellular IL-10 and CCL8 Secretion during latent infection through changes in the cellular microRNA hsa-miR-92a. Poole E, Avdic S, Hodkinson J, Jackson S, Wills M, Slobedman B, Sinclair J. J Virol. 2014 Dec;88(24):13947-55. doi: 10.1128/JVI.02424-14. Epub 2014 Sep 24

Human cytomegalovirus latency-associated proteins elicit immune-suppressive IL-10 producing CD4⁺ T cells. Mason GM, Jackson S, Okecha G, Poole E, Sissons JG, Sinclair J, Wills MR. PLoS Pathog. 2013;9(10):e1003635. doi: 10.1371/journal.ppat.1003635. Epub 2013 Oct 10.

Human cytomegalovirus gene UL76 induces IL-8 expression through activation of the DNA damage response. Costa H, Nascimento R, Sinclair J, Parkhouse RM. PLoS Pathog. 2013 Sep;9(9):e1003609. doi: 10.1371/journal.ppat.1003609. Epub 2013 Sep 12.

Circulating dendritic cells isolated from healthy seropositive donors are sites of human cytomegalovirus reactivation in vivo. Reeves MB, Sinclair JH. J Virol. 2013 Oct;87(19):10660-7. doi: 10.1128/JVI.01539-13. Epub 2013 Jul 24.

Regulation of human cytomegalovirus transcription in latency: beyond the major immediate-early promoter. Reeves M, Sinclair J. Viruses. 2013 Jun 3;5(6):1395-413. doi: 10.3390/v5061395. Review.

Latency-associated degradation of the MRP1 drug transporter during latent human cytomegalovirus infection. Weekes MP, Tan SY, Poole E, Talbot S, Antrobus R, Smith DL, Montag C, Gygi SP, Sinclair JH, Lehner PJ Science. 2013 Apr 12;340(6129):199-202. doi: 10.1126/science.1235047.

The myeloid transcription factor GATA-2 regulates the viral UL144 gene during human cytomegalovirus latency in an isolate-specific manner. Poole E, Walther A, Raven K, Benedict CA, Mason GM, Sinclair J. J Virol. 2013 Apr;87(8):4261-71. doi: 10.1128/JVI.03497-12. Epub 2013 Jan 30.

The cellular protein MCM3AP is required for inhibition of cellular DNA synthesis by the IE86 protein of human cytomegalovirus. Poole E, Bain M, Teague L, Takei Y, Laskey R, Sinclair J. PLoS One. 2012;7(10):e45686. doi: 10.1371/journal.pone.0045686. Epub 2012 Oct 19.

Human cytomegalovirus latency alters the cellular secretome, inducing cluster of differentiation (CD)4+ T-cell migration and suppression of effector function. Mason GM, Poole E, Sissons JG, Wills MR, Sinclair JH. Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14538-43. doi: 10.1073/pnas.1204836109. Epub 2012 Jul 23.

Depletion of cellular pre-replication complex factors results in increased human cytomegalovirus DNA replication. Braun TE, Poole E, Sinclair J. PLoS One. 2012;7(5):e36057. doi: 10.1371/journal.pone.0036057. Epub 2012 May 7.

 

Wei-Li Kuan, Emma Poole, Michael Fletcher, Sharon Karniely, Pam Tyers, Mark Wills, Roger A. Barker, and John H. Sinclair (2011) A novel neuroprotective therapy for Parkinson’s disease using a viral non-coding RNA that protects mitochondrial Complex I activity J. Experimental Medicine Jan 16;209(1):1-10

Emma Poole, Stuart R. McGregor Dallas, Julia Colston, Robert Samuel V. Joseph and John Sinclair Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34+ progenitors (2011) Virally induced changes in cellular miRNAs maintain latency of human cytomegalovirus in CD34+ progenitors. J Gen Virol. 92:1539-1549

Reeves M, Woodhall D, Compton T, Sinclair J (2010) Human cytomegalovirus IE72 protein interacts with the transcriptional repressor hDaxx to regulate LUNA gene expression during lytic infection. J Virol.  84(14): 7185-94

J. Sinclair, Chromatin structure regulates human cytomegalovirus gene expression during latency, reactivation and lytic infection (2010) Biochim Biophys Acta 1799:286-95.

Poole E, Groves I, MacDonald A, Pang Y, Alcami A, Sinclair J (2009) Identification of TRIM23 as a cofactor involved in the regulation of NF-kappaB by human cytomegalovirus J Virol. 83(8):3581-90

Poole E, Atkins E, Nakayama T, Yoshie O, Groves I, Alcami A, Sinclair J (2008) NF-kappaB-mediated activation of the chemokine CCL22 by the product of the human cytomegalovirus gene UL144 escapes regulation by viral IE86 J Virol. 82(9): 4250-6

Sinclair, J. (2008) Human cytomegalovirus: Latency and reactivation in the myeloid lineage. J Clin. Virol:. 41:180-185

Reeves, MB, Davies, AA, McSharry, BP, Wilkinson, GW and Sinclair JH (2007) Complex I binding by a virally encoded RNA regulates mitochondria-induced cell death. Science 316:1345-1348.

Goodrum, FD, Reeves, M, Sinclair J, High, K. and Shenk, T (2007) Human cytomegalovirus sequences expressed in latently infected individuals promote a latent infection in vitro Blood 110:937-945

Matthew Reeves, Paul MacAry, Paul Lehner, Patrick Sissons and John Sinclair (2005) Latency, Chromatin Remodelling and Reactivation of Human Cytomegalovirus in the Dendritic Cells of Healthy Carriers PNAS 102:4140-4145

Poole, E, King, C, Sinclair, J and Alcami, A (2006) The UL144 gene product of human cytomegalovirus activates NFkappaB via a TRAF6-dependent mechanism. EMBO J 18: 4390-4399