The Immunophenotyping Hub contains state of the art cell sorters, FACS analysers and confocal microscopes. We are housed on the 6th floor extension to the Department of Medicine lab-block (e6)

Our 3 main cell sorters comprise a 15 colour AriaIII-Fusion, a 13 colour AriaIII and a 16 colour BD Influx, all contained inside custom cat2 hoods, allowing us to sort unscreened patient material. We also have a 6 colour FACSJazz for simpler Cat1 sorts.

All users must receive specific training on all confocals and analysers. For facility induction contact Anna Petrunkina, for all bookings contact our listserv

Facility Status

machine status qc date charge data purge
Fortessa analyser OK 230315 £21 p/h scheduled feb 16th-2015
Fortessa2 analyser OK 240315 £21 p/h scheduled feb 16th-2015
CantoII analyser OK 230315 £21 p/h scheduled feb 16th-2015
FacsCalibur analyser OK 230315 £21 p/h scheduled feb 16th-2015
AriaIII cell sorter OK 230315 £72 p/h n/a
Aria-fusion OK 230315 £72 p/h n/a
Influx testing testing £72 p/h n/a
FACSJazz cell sorter OK 230315 £72 p/h n/a
Leica SPE confocal OK 031013 £21 p/h scheduled feb 16th-2015
Leica Sp5 confocal OK July 2013 £21 p/h scheduled feb 16th-2015
Zeiss Axioskop ‘scope OK 250712 £free n/a
MS3 Coulter Counter OK 230215 £free n/a
AutoMACSPro OK 250315 £48+£30 p/h n/a
       
Data Clearance, Analysers and Confocals

Just a final reminder that the next regular clear up of hard drives on analysers and microscopes is due begin on 16th February 2015.
From this point, all data that have been acquired before 31st December 2014 will be gradually deleted and the hard drives on analysers and microscopes cleared up.
Please make sure that all data are backed up – they will be removed irreversibly.
If you have any concerns or queries, or somebody from your group is away on extended leave and cannot back up their data, please talk to the Hub staff NOW to extend their deadline.

4th UK Cytometry Core Managers Forum

20th April 2015; Cambridge Institute for Medical Research, Addenbrooke’s BioMedical Campus

Download Flyer

Please register with Reiner Shulte

more “BuildingCyt” Modular Training Courses

Module 1 (single day)

5th June, 3rd September; Royal Cambridge Hotel

3rd September 2015; Babraham Research Campus

1st October 2015; Babraham Research Campus

Module 2 (2 days)

22nd and 23rd July 2015; Babraham Research Campus

22nd and 23rd October 2015; Babraham Research Campus

register to flow_training@babraham.ac.uk

Immunophenotyping Service Announcement
Our BRC Cell Phenotyping Hub offers Immunophenotyping services to Cambridge BRC members, including panel assessments on the basic (analysis), comprehensive (analysis and cell separation) and advanced (purification of phenotyped cells)

Packages (per sample/assessment/half-day instrument use ; surcharge applied for extra time, additional antibodies and MACS beads scaled up to the number of cells)

Basic Phenotyping Assessment £190

Comprehensive Phenotyping Assessment and Cell Separation £300

Comprehensive Phenotyping and Fluorescence Activated Cell Sorting £425

These services can be customised to individual needs such that services can be used instead of packages full details of service (updated 031114)

Immunophenotyping Service Announcement
* Fortessa base configuration change on the 6th October 2014: this is to accommodate an extra detector on the violet laser, but all new experiments will need to reset configuration (The master list of the names of the detectors). Hub staff are available to help during the change.
New Risk Assessment for cat2 conversion of analyser room updated 070513
Hub policy Latest statement of Hub policy-291112
CRI Amnis image stream use Use of this imaging cytometer available through Richard Grenfell The machine is housed in the CRI building on site at Addenbrooke’s