NIHR Cambridge BRC Cell Phenotyping Hub


The Immunophenotyping Hub contains state of the art cell sorters, FACS analysers and confocal microscopes. We are housed on the 6th floor extension to the department of medicine lab-block (e6)

Our Primary cell sorter is a 13 colour AriaIII that is contained inside a custom built cat2 hood, allowing us to sort unscreened patient material

All users must receive specific training on all confocals and analysers. For facility induction contact Anna Petrunkina, for all bookings contact Simon McCallum

machine status qc date charge data purge
Fortessa analyser OK 210414 £20 p/h completed june-2013
CantoII analyser OK 210414 £20 p/h completed june-2013
FacsCalibur analyser OK 210414 £20 p/h n/a
AriaIII cell sorter OK 210414 £70 p/h n/a
Aria-fusion OK 210414 £70 p/h n/a
FACSJazz cell sorter OK 240314 £70 p/h n/a
Leica SPE confocal OK 031013 £20 p/h completed june-2013
Leica Sp5 confocal OK July 2013 £20 p/h completed june-2013
Zeiss Axioskop ‘scope OK 250712 £free n/a

1st in series of talks on Advanced Flow Cytometry: Cell Cycle and Apoptosis Thursday 24th April, 10-11.30 am, CRI Lecture Theatre Speaker: Richard Grenfell
TReg/THelper symposium 25th April 2014 (programme) Royal Veterinary College, Camden Campus, London
Meeting focus: autoimmune disease and cancer (d/load flyer)
Starlab Pipette Clinic, 28th to 30th April £11 for a single channel pipette, £24 for a 8 channel
2nd in series of talks on Advanced Flow Cytometry: Intracellular cytokine staining 7th May, 10-11.00 am, Sanger room, newLMB Speaker: Maria Daly
“Screening in suspension” Roadshow from Intellicyte Monday 12th May, Little Chesterford, nr. Saffron Waldon
RMS Residential Flow Cytometry Course organised by Peter O’Toole 15th to 19th September 2014, University of York
New Risk Assessment for cat2 conversion of analyser room updated 070513
New Policy Statement / Increase of charges from 1st April 2013 Analyser/Confocal charges increased to £20 p/h, AriaIII/cat2 sorts charged at £70 p/h
Hub policy Latest statement of Hub policy-291112
CRI Amnis image stream use Use of this imaging cytometer available through Richard Grenfell The machine is housed in the CRI building on site at Addenbrooke’s