The Immunophenotyping Hub contains state of the art cell sorters, FACS analysers and confocal microscopes. We are housed on the 6th floor extension to the Department of Medicine lab-block (e6)

Our 3 main cell sorters comprise a 15 colour AriaIII-Fusion, a 13 colour AriaIII and a 16 colour BD Influx, all contained inside custom cat2 hoods, allowing us to sort unscreened patient material. We also have a 6 colour 3 laser FACSJazz for simpler Cat1 sorts.

All new users of our analysers and microscopes must receive an induction to the facility and specific training for each machine. All new clients of our sorting facility also require a consultation.

For facility induction/consultation contact Anna Petrunkina; for all bookings contact our listserv

Facility Status

machine status qc date charge data purge
Fortessa analyser OK 170815 £21 p/h completed
Fortessa2 analyser OK 170815 £21 p/h completed
CantoII analyser OK 170815 £21 p/h completed
FacsCalibur analyser OK 240715 £21 p/h completed
AriaIII cell sorter OK 170815 £72 p/h n/a
Aria-fusion OK 170815 £72 p/h n/a
Influx OK 170815 £72 p/h n/a
FACSJazz cell sorter OK 230715 £72 p/h n/a
Leica SPE confocal OK June 2015 £21 p/h completed
Leica Sp5 confocal fixed! 170815 £21 p/h completed
Zeiss Axioskop ‘scope OK 250712 £free n/a
MS3 Coulter Counter OK 230215 £free n/a
AutoMACSPro OK 210715 £48+£30 p/h n/a
       
Advanced notice of refurbishments in E6

All data that have been acquired before 1st July on analysers and microscopes and before 1st August on sorters will be gradually deleted. Please make sure that all data have been backed up – they will be removed irreversibly. If you have any concerns or queries, or if somebody from your group is away on leave and cannot back up their data, please talk to the Hub staff in advance to extend their deadline.

 
Final Reminder of data deletion

All data that have been acquired before 1st July on analysers and microscopes and before 1st August on sorters will be gradually deleted. Please make sure that all data have been backed up – they will be removed irreversibly. If you have any concerns or queries, or if somebody from your group is away on leave and cannot back up their data, please talk to the Hub staff in advance to extend their deadline.

Reminder
All new users of analysers and /or microscopes and all new clients of our sorting facility must have an induction with our director: Anna Petrunkina
 
London Flow Club
Wednesday 23rd September, Rayne Building, UCL, University, Drinks from 6pm, more details following soon
 
RMS Advances in Cytometry Meeting, Charles Darwin House, London UK
13th November 2015, Programme available from weblink

 
RMS Residential Flow Cytometry Course

13th to 18th September 2015; York University

Download Flyer

more “BuildingCyt” Modular Training Courses

Module 1 (single day)

5th June, 3rd September; Royal Cambridge Hotel

3rd September 2015; Babraham Research Campus

1st October 2015; Babraham Research Campus

Module 2 (2 days)

22nd and 23rd July 2015; Babraham Research Campus

22nd and 23rd October 2015; Babraham Research Campus

register to flow_training@babraham.ac.uk

Immunophenotyping Service Announcement
Our BRC Cell Phenotyping Hub offers Immunophenotyping services to Cambridge BRC members, including panel assessments on the basic (analysis), comprehensive (analysis and cell separation) and advanced (purification of phenotyped cells)

Packages (per sample/assessment/half-day instrument use ; surcharge applied for extra time, additional antibodies and MACS beads scaled up to the number of cells)

Basic Phenotyping Assessment £190

Comprehensive Phenotyping Assessment and Cell Separation £300

Comprehensive Phenotyping and Fluorescence Activated Cell Sorting £425

These services can be customised to individual needs such that services can be used instead of packages full details of service (updated 090415)

Immunophenotyping Service Announcement
* Fortessa base configuration change on the 6th October 2014: this is to accommodate an extra detector on the violet laser, but all new experiments will need to reset configuration (The master list of the names of the detectors). Hub staff are available to help during the change.
New Risk Assessment for cat2 conversion of analyser room updated 010814
Hub policy Latest statement of Hub policy-291112
CRI Amnis image stream use Use of this imaging cytometer available through Richard Grenfell The machine is housed in the CRI building on site at Addenbrooke’s