The Immunophenotyping Hub contains state of the art cell sorters, FACS analysers and confocal microscopes. We are housed on the 6th floor extension to the Department of Medicine lab-block (e6)
Our 3 main cell sorters comprise a 15 colour AriaIII-Fusion, a 13 colour AriaIII and a 16 colour BD Influx, all contained inside custom cat2 hoods, allowing us to sort unscreened patient material. We also have a 6 colour FACSJazz for simpler Cat1 sorts.
All new users of our analysers and microscopes must receive an induction to the facility and specific training for each machine. All new clients of our sorting facility also require a consultation.
|machine||status||qc date||charge||data purge|
|Fortessa analyser||OK||220615||£21 p/h||completed|
|Fortessa2 analyser||OK||220615||£21 p/h||completed|
|CantoII analyser||OK||220615||£21 p/h||completed|
|FacsCalibur analyser||OK||220615||£21 p/h||completed|
|AriaIII cell sorter||OK||220615||£72 p/h||n/a|
|FACSJazz cell sorter||OK||220615||£72 p/h||n/a|
|Leica SPE confocal||out of action||031014||£21 p/h||completed|
|Leica Sp5 confocal||OK||June 2015||£21 p/h||completed|
|Zeiss Axioskop ‘scope||OK||250712||£free||n/a|
|MS3 Coulter Counter||OK||230215||£free||n/a|
|Skeleton service in late June|
Due to refurbishments and staff absence, only a skeleton service will be available on 25th-30th June.
|Advanced notice of refurbishments in E6|
First phase of works will start on or after 15th June, and will be limited to a new room that will house new equipment. Thus, no inconvenience will be caused to the users of the Hub although some noise and increased public traffic is expected.
The second phase of works is due to commence on 25th June in the sorting room and last until 30th June. The sorting operations will continue but availability will be reduced.
The third phase of works will commence in w/c 29th June and cover installation of an air conditioning system in the left half of the analyser room. The works are likely to last about two days and will cause noise and dust. Although access to analysers will remain open, it would be advisable to avoid lengthy operations during core hours.
The fourth phase of works will commence in w/c 7th July and cover electrics and wall works in sorting room. That is expected to last 3-4 days, and will limit availability to one sorter
The final stage of works will be conducted in the analyser room. The dates of these works are undefined yet as estates have to conduct an asbestos survey first.
Thank you for your understanding and please let us know if you have any queries or concerns. We apologise in advance for any inconvenience associated with the refurbishments and for any unplanned developments.
|BD Horizons tour: Cambridge|
By popular demand, we are hosting a Horizon Tour, focussing on developing skills and insights into multicolour panel design. The event will consist of seminar series and address the panel design process.
This event will be organised in cooperation with BD Bioscience and is accessible to all our users and collaborators.
The event is suitable for both experienced and unexperienced individuals pursuing research utilising flow and phenotyping tools, and we hope it will benefit the research and education in the Clinical School and across the Biomedical Research Campus
Please let the hub know if you have any queries or issues with the registration. The numbers are limited at the moment to booking on the first come, first served basis, but we will make an effort to accommodate more attendees if the interest is expressed early.
We are about to upgrade our old Aria III with an expanded choice of violet laser detectors. This upgrade will essentially bring the machine up to the standard of the AriaFusion.
The first stage of the upgrade is scheduled on 30th of June, therefore there will be resticted availability around that time
Unfortunately, after the change of workstation and software, existing templates and settings will become invalid and will have to be reconstructed, so please bring a full panel of single-stained controls on your first bookings in July. If you need to archive data that is currently on the ariaIII, please contact hub staff.
|All new users of analysers and /or microscopes and all new clients of our sorting facility must have an induction with our director: Anna Petrunkina|
|London Flow Club|
|Wednesday 8th July from 6pm
The Rayne Building, University College London, University Street.
Please contact Jamie Evans to register for security purposes
|RMS Residential Flow Cytometry Course
13th to 18th September 2015; York University
Please register at this page
|more “BuildingCyt” Modular Training Courses
Module 1 (single day)
5th June, 3rd September; Royal Cambridge Hotel
3rd September 2015; Babraham Research Campus
1st October 2015; Babraham Research Campus
Module 2 (2 days)
22nd and 23rd July 2015; Babraham Research Campus
22nd and 23rd October 2015; Babraham Research Campus
|Immunophenotyping Service Announcement
Our BRC Cell Phenotyping Hub offers Immunophenotyping services to Cambridge BRC members, including panel assessments on the basic (analysis), comprehensive (analysis and cell separation) and advanced (purification of phenotyped cells)
Packages (per sample/assessment/half-day instrument use ; surcharge applied for extra time, additional antibodies and MACS beads scaled up to the number of cells)
Basic Phenotyping Assessment £190
Comprehensive Phenotyping Assessment and Cell Separation £300
Comprehensive Phenotyping and Fluorescence Activated Cell Sorting £425
These services can be customised to individual needs such that services can be used instead of packages full details of service (updated 090415)
|Immunophenotyping Service Announcement
* Fortessa base configuration change on the 6th October 2014: this is to accommodate an extra detector on the violet laser, but all new experiments will need to reset configuration (The master list of the names of the detectors). Hub staff are available to help during the change.
|New Risk Assessment for cat2 conversion of analyser room||updated 010814|
|Hub policy||Latest statement of Hub policy-291112|
|CRI Amnis image stream use||Use of this imaging cytometer available through Richard Grenfell The machine is housed in the CRI building on site at Addenbrooke’s|