The NIHR Cambridge BRC Cell Phenotyping Hub is an important part of Cambridge BRC infrastructure and, simultaneously, is a part of the cross-cutting Inflammation, Infection and Immune-therapeutics research theme. The Hub delivers advanced scientific technologies supporting the excellence in research for a partnership between the University of Cambridge and Cambridge University Hospitals NHS Foundation Trust.
The Hub’s mission is to provide state-of-the-art resources and cost-effective high quality scientific, educational and technological services across the wide range of cytomics. These services fall mainly into three major areas: research, clinical and technology services and include (but are not limited to) cell and tissue processing, cell isolation (sorting), cell analysis, cell and tissue imaging, and cell phenotyping. A further function of the Hub is to collect and to disseminate the unique technological and scientific expertise crucial for promoting and supporting patient-based basic research and for translating basic biological findings into clinical practice. The Hub drives technological innovations and applies technical and instrumental advances to expand the portfolio of services and fulfil the emerging research needs. Through our collaborative Research and Development service we foster and fortify collaborations that result in many major publications.
The Hub is equipped with state-of the art equipment including high speed cell sorters, bench top analysers, microscopes and high content/high throughput equipment.
The Hub laboratories are located on Level 6 of the Addenbrooke’s main hospital block in E6. For access to the Hub services, all new clients and prospective collaborators are invited to attend a Hub induction with our Director (apl. Professor Anna Petrunkina-Harrison) to discuss their research needs and to customise the provision of services. Project-focussed consultations, theory courses and practical tutorials on instruments can be arranged after completing the induction. For all the bookings, cancellations and reporting any technical problems please contact our urgent enquiries mailing list.
|Machine||Status||QC date||Charge||Data Purge|
|Fortessa analyser||OK||290118||£26 p/h||Done|
|Fortessa2 analyser||OK||290118||£26 p/h||Done|
|CantoII analyser||OK||290118||£26 p/h||Done|
|FacsCalibur analyser||OK||220118||£26 p/h||Done|
|Attune NxT||OK||220118||£26 p/h||n/a|
|Cellomics XTI||OK||200917||£26 p/h||n/a|
|AriaIII cell sorter||OK||011217||£81 p/h||Done|
|FACSJazz cell sorter||OK||090118||£81 p/h||Done|
|Melody Cell Sorter||OK||290118||£39||n/a|
|Leica SPE confocal||OK||210617||£26 p/h||Done|
|Leica Sp5 confocal||OK||140617||£26 p/h||Done|
|Leica LMD6||OK||060516||£26 p/h||n/a|
|Seahorse XF96||OK||n/a||£35 p/h||n/a|
|Zeiss Axioskop ‘scope||OK||250712||£free||n/a|
News and Events
|Flow Cytometry Assistant Position in CIMR|
|Applicants are welcome to contact Reiner Shulte (Manager of CIMR Flow Cytometry) before application|
|BD Biosciences Discovery Day|
|Wednesday 21st March all day starting 9.45am, De Vere Holborn Bars, Central London|
|BD Biosciences FACSymphony™ Seminar, [Rescheduled!]|
|Contact Maria Daly for Registration Thursday the 22nd February 2018, 10am, The Milstein Room, LMB, Francis Crick Avenue.|
|New Brilliant UV dyes available|
|There now are six different Brilliant UV dyes available. 30% discount available through BD Biosciences at this link, which times out on 28th February 2018|
|BD Biosciences Discovery Day, 21st March 2018|
|10am to 4pm, De Vere Holborn Bars, Central London|
|Mass Cytometry Course at King’s College, London|
|The course will run over five days from 19th to the 23rd March 2018||Content will cover practical aspects, tools for high dimensional data analysis and presentations on various practical aspects of using mass cytometry for research and clinical applications. Delegates have a choice of attending either one of three modules (practical only, data analytics only or full course).|
|London Flow Club|
|1st March 2018, 6pm Institute of Cancer Research. Sponsored by Merck Millipore. Talks from David Mansfield on “Vectra Multiplex fIHC on FFPE tissue” and Kate Bergerhoff: “Applications of Flow Cytometry in Immunotherapy of Thyroid Carcinomas” and PJ Chana on “CCD cameras in Imaging Flow Cytometry”|
|Please contact Fredrik Wallberg for Security Clearance|
|Price increases form 1st August 2017|
On 1st August 2017 all Hub user charges will increase to account for anticipated higher operational costs. The anticipated charges should be used for grant costing from this date. These figures have already been communicated to all Heads of Departments of the Clinical School.
The hub will still continue to operate at below average end-user cost considered nationally and/or locally.
The Hub depends on BRC funding together with capital equipment grants. In order to maintain and expand this infrastructure we need to demonstrate the relevance of our facilities, services and expertise in Scientific Publication.
|Babraham Flow Courses 2018: Module 1 (Fundamentals)|
|Available dates are 28th March 2018, 29th March 2018, 20th September 2018 and 21st September|
|Babraham Flow Course 2018: Module 2 (Advanced 2 day course)|
|Available dates are 18th and 19th April 2018;
23rd and 24th April; 9th and 10th October 2018