The Immunophenotyping Hub contains state of the art cell sorters, FACS analysers and confocal microscopes. We are housed on the 6th floor extension to the Department of Medicine lab-block (e6)

Our 3 main cell sorters comprise a 15 colour AriaIII-Fusion, a 13 colour AriaIII and a 16 colour BD Influx, all contained inside custom cat2 hoods, allowing us to sort unscreened patient material. We also have a 6 colour FACSJazz for simpler Cat1 sorts.

All new users of our analysers and microscopes must receive an induction to the facility and specific training for each machine. All new clients of our sorting facility also require a consultation.

For facility induction/consultation contact Anna Petrunkina; for all bookings contact our listserv

Facility Status

machine status qc date charge data purge
Fortessa analyser OK 220615 £21 p/h completed
Fortessa2 analyser OK 220615 £21 p/h completed
CantoII analyser OK 220615 £21 p/h completed
FacsCalibur analyser OK 220615 £21 p/h completed
AriaIII cell sorter OK 220615 £72 p/h n/a
Aria-fusion OK 220615 £72 p/h n/a
Influx OK 220615 £72 p/h n/a
FACSJazz cell sorter OK 220615 £72 p/h n/a
Leica SPE confocal out of action 031014 £21 p/h completed
Leica Sp5 confocal OK June 2015 £21 p/h completed
Zeiss Axioskop ‘scope OK 250712 £free n/a
MS3 Coulter Counter OK 230215 £free n/a
AutoMACSPro OK 280415 £48+£30 p/h n/a
       
Skeleton service in late June

Due to refurbishments and staff absence, only a skeleton service will be available on 25th-30th June.

 
Advanced notice of refurbishments in E6

First phase of works will start on or after 15th June, and will be limited to a new room that will house new equipment. Thus, no inconvenience will be caused to the users of the Hub although some noise and increased public traffic is expected.

The second phase of works is due to commence on 25th June in the sorting room and last until 30th June. The sorting operations will continue but availability will be reduced.

The third phase of works will commence in w/c 29th June and cover installation of an air conditioning system in the left half of the analyser room. The works are likely to last about two days and will cause noise and dust. Although access to analysers will remain open, it would be advisable to avoid lengthy operations during core hours.

The fourth phase of works will commence in w/c 7th July and cover electrics and wall works in sorting room. That is expected to last 3-4 days, and will limit availability to one sorter

The final stage of works will be conducted in the analyser room. The dates of these works are undefined yet as estates have to conduct an asbestos survey first.

Thank you for your understanding and please let us know if you have any queries or concerns. We apologise in advance for any inconvenience associated with the refurbishments and for any unplanned developments.

 
BD Horizons tour: Cambridge

By popular demand, we are hosting a Horizon Tour, focussing on developing skills and insights into multicolour panel design. The event will consist of seminar series and address the panel design process.

It will take place on 14th July in the lecture Theatre of Clifford Albutt Building

This event will be organised in cooperation with BD Bioscience and is accessible to all our users and collaborators.

The event is free but the registration is required

The event is suitable for both experienced and unexperienced individuals pursuing research utilising flow and phenotyping tools, and we hope it will benefit the research and education in the Clinical School and across the Biomedical Research Campus

Please let the hub know if you have any queries or issues with the registration. The numbers are limited at the moment to booking on the first come, first served basis, but we will make an effort to accommodate more attendees if the interest is expressed early.

 
AriaIII upgrade

We are about to upgrade our old Aria III with an expanded choice of violet laser detectors. This upgrade will essentially bring the machine up to the standard of the AriaFusion.

The first stage of the upgrade is scheduled on 30th of June, therefore there will be resticted availability around that time

Unfortunately, after the change of workstation and software, existing templates and settings will become invalid and will have to be reconstructed, so please bring a full panel of single-stained controls on your first bookings in July. If you need to archive data that is currently on the ariaIII, please contact hub staff.

 
Reminder
All new users of analysers and /or microscopes and all new clients of our sorting facility must have an induction with our director: Anna Petrunkina
 
London Flow Club
Wednesday 8th July from 6pm
The Rayne Building, University College London, University Street.
Please contact Jamie Evans to register for security purposes

Download flyer

 
RMS Residential Flow Cytometry Course

13th to 18th September 2015; York University

Download Flyer

Please register at this page

more “BuildingCyt” Modular Training Courses

Module 1 (single day)

5th June, 3rd September; Royal Cambridge Hotel

3rd September 2015; Babraham Research Campus

1st October 2015; Babraham Research Campus

Module 2 (2 days)

22nd and 23rd July 2015; Babraham Research Campus

22nd and 23rd October 2015; Babraham Research Campus

register to flow_training@babraham.ac.uk

Immunophenotyping Service Announcement
Our BRC Cell Phenotyping Hub offers Immunophenotyping services to Cambridge BRC members, including panel assessments on the basic (analysis), comprehensive (analysis and cell separation) and advanced (purification of phenotyped cells)

Packages (per sample/assessment/half-day instrument use ; surcharge applied for extra time, additional antibodies and MACS beads scaled up to the number of cells)

Basic Phenotyping Assessment £190

Comprehensive Phenotyping Assessment and Cell Separation £300

Comprehensive Phenotyping and Fluorescence Activated Cell Sorting £425

These services can be customised to individual needs such that services can be used instead of packages full details of service (updated 090415)

Immunophenotyping Service Announcement
* Fortessa base configuration change on the 6th October 2014: this is to accommodate an extra detector on the violet laser, but all new experiments will need to reset configuration (The master list of the names of the detectors). Hub staff are available to help during the change.
New Risk Assessment for cat2 conversion of analyser room updated 010814
Hub policy Latest statement of Hub policy-291112
CRI Amnis image stream use Use of this imaging cytometer available through Richard Grenfell The machine is housed in the CRI building on site at Addenbrooke’s