NIHR Cambridge BRC Cell Phenotyping Hub


The Immunophenotyping Hub contains state of the art cell sorters, FACS analysers and confocal microscopes. We are housed on the 6th floor extension to the department of medicine lab-block (e6)

Our 2 Primary cell sorters are BD-AriaIIIs, housed inside custom built cat2 hoods. This allows us to sort unscreened patient material

All users must receive specific training on all confocals and analysers. For facility induction contact Anna Petrunkina, for all bookings contact all e6 staff listserv

machine status qc date charge data purge
Fortessa analyser fixed! 151214 £21 p/h scheduled october-2014
Fortessa2 analyser OK 151214 £21 p/h scheduled october-2014
CantoII analyser OK 151214 £21 p/h scheduled october-2014
FacsCalibur analyser OK 151214 £21 p/h scheduled october-2014
AriaIII cell sorter OK 151214 £72 p/h n/a
Aria-fusion OK 151214 £72 p/h n/a
FACSJazz cell sorter OK 011214 £72 p/h n/a
Leica SPE confocal OK 140814 £21 p/h scheduled october-2014
Leica Sp5 confocal OK 300714 £21 p/h scheduled october-2014
Zeiss Axioskop ‘scope OK 250712 £free n/a
MS3 Coulter Counter OK 281014 £free n/a

“BuildingCyt” Modular Training Courses

One day ‘Fundamentals of Flow Cytometry’

6th and 18th March 2015

also Two day ‘Advanced Flow Cytometry’ course

19th and 20th March 2015

register to

Immunophenotyping Service Announcement
Our BRC Cell Phenotyping Hub offers Immunophenotyping services to Cambridge BRC members, including panel assessments on the basic (analysis), comprehensive (analysis and cell separation) and advanced (purification of phenotyped cells)

Packages (per sample/assessment/half-day instrument use ; surcharge applied for extra time, additional antibodies and MACS beads scaled up to the number of cells)

Basic Phenotyping Assessment £190

Comprehensive Phenotyping Assessment and Cell Separation £300

Comprehensive Phenotyping and Fluorescence Activated Cell Sorting £425

These services can be customised to individual needs such that services can be used instead of packages full details of service (updated 031114)

Immunophenotyping Service Announcement
* Fortessa base configuration change on the 6th October 2014: this is to accommodate an extra detector on the violet laser, but all new experiments will need to reset configuration (The master list of the names of the detectors). Hub staff are available to help during the change.
New Risk Assessment for cat2 conversion of analyser room updated 070513
Flow Cytometry Course at York University 27th to 30th January 2015
Hub policy Latest statement of Hub policy-291112
CRI Amnis image stream use Use of this imaging cytometer available through Richard Grenfell The machine is housed in the CRI building on site at Addenbrooke’s