The Immunophenotyping Hub contains state of the art cell sorters, FACS analysers and confocal microscopes. We are housed on the 6th floor extension to the Department of Medicine lab-block (e6)
Our 3 main cell sorters comprise a 15 colour AriaIII-Fusion, a 13 colour AriaIII and a 16 colour BD Influx, all contained inside custom cat2 hoods, allowing us to sort unscreened patient material. We also have a 6 colour 3 laser FACSJazz for simpler Cat1 sorts.
All new users of our analysers and microscopes must receive an induction to the facility and specific training for each machine. All new clients of our sorting facility also require a consultation.
|machine||status||qc date||charge||data purge|
|Fortessa analyser||OK||240715||£21 p/h||completed|
|Fortessa2 analyser||fixed!||230715||£21 p/h||completed|
|CantoII analyser||OK||240715||£21 p/h||completed|
|FacsCalibur analyser||OK||240715||£21 p/h||completed|
|AriaIII cell sorter||OK||240715||£72 p/h||n/a|
|FACSJazz cell sorter||OK||230715||£72 p/h||n/a|
|Leica SPE confocal||OK||June 2015||£21 p/h||completed|
|Leica Sp5 confocal||out of action||June 2015||£21 p/h||completed|
|Zeiss Axioskop ‘scope||OK||250712||£free||n/a|
|MS3 Coulter Counter||OK||230215||£free||n/a|
|Advanced notice of refurbishments in E6|
The fourth phase of works will commence in w/c 7th July and cover electrics and wall works in sorting room. That is expected to last 3-4 days, and will limit availability to one sorter
The final stage of works will be conducted in the analyser room. The dates of these works are undefined yet as estates have to conduct an asbestos survey first.
Thank you for your understanding and please let us know if you have any queries or concerns. We apologise in advance for any inconvenience associated with the refurbishments and for any unplanned developments.
We are about to upgrade our old Aria III with an expanded choice of violet laser detectors. This upgrade will essentially bring the machine up to the standard of the AriaFusion.
The first stage of the upgrade is scheduled on 30th of June, therefore there will be resticted availability around that time
Unfortunately, after the change of workstation and software, existing templates and settings will become invalid and will have to be reconstructed, so please bring a full panel of single-stained controls on your first bookings in July. If you need to archive data that is currently on the ariaIII, please contact hub staff.
|All new users of analysers and /or microscopes and all new clients of our sorting facility must have an induction with our director: Anna Petrunkina|
|RMS Residential Flow Cytometry Course
13th to 18th September 2015; York University
Please register at this page
|more “BuildingCyt” Modular Training Courses
Module 1 (single day)
5th June, 3rd September; Royal Cambridge Hotel
3rd September 2015; Babraham Research Campus
1st October 2015; Babraham Research Campus
Module 2 (2 days)
22nd and 23rd July 2015; Babraham Research Campus
22nd and 23rd October 2015; Babraham Research Campus
|Immunophenotyping Service Announcement
Our BRC Cell Phenotyping Hub offers Immunophenotyping services to Cambridge BRC members, including panel assessments on the basic (analysis), comprehensive (analysis and cell separation) and advanced (purification of phenotyped cells)
Packages (per sample/assessment/half-day instrument use ; surcharge applied for extra time, additional antibodies and MACS beads scaled up to the number of cells)
Basic Phenotyping Assessment £190
Comprehensive Phenotyping Assessment and Cell Separation £300
Comprehensive Phenotyping and Fluorescence Activated Cell Sorting £425
These services can be customised to individual needs such that services can be used instead of packages full details of service (updated 090415)
|Immunophenotyping Service Announcement
* Fortessa base configuration change on the 6th October 2014: this is to accommodate an extra detector on the violet laser, but all new experiments will need to reset configuration (The master list of the names of the detectors). Hub staff are available to help during the change.
|New Risk Assessment for cat2 conversion of analyser room||updated 010814|
|Hub policy||Latest statement of Hub policy-291112|
|CRI Amnis image stream use||Use of this imaging cytometer available through Richard Grenfell The machine is housed in the CRI building on site at Addenbrooke’s|