The Immunophenotyping Hub contains state of the art cell sorters, FACS analysers and confocal microscopes. We are housed on the 6th floor extension to the Department of Medicine lab-block (e6)

Our 3 main cell sorters comprise a 15 colour AriaIII-Fusion, a 13 colour AriaIII and a 16 colour BD Influx, all contained inside custom cat2 hoods, allowing us to sort unscreened patient material. We also have a 6 colour FACSJazz for simpler Cat1 sorts.

All new users of our analysers and microscopes must receive an induction to the facility and specific training for each machine. All new clients of our sorting facility also require a consultation.

For facility induction/consultation contact Anna Petrunkina; for all bookings contact our listserv

Facility Status

machine status qc date charge data purge
Fortessa analyser fixed 180515 £21 p/h completed
Fortessa2 analyser OK 130515 £21 p/h completed
CantoII analyser OK 110515 £21 p/h completed
FacsCalibur analyser OK 110515 £21 p/h completed
AriaIII cell sorter OK 110515 £72 p/h n/a
Aria-fusion OK 110515 £72 p/h n/a
Influx OK 110515 £72 p/h n/a
FACSJazz cell sorter OK 110515 £72 p/h n/a
Leica SPE confocal OK 031014 £21 p/h completed
Leica Sp5 confocal OK July 2014 £21 p/h completed
Zeiss Axioskop ‘scope OK 250712 £free n/a
MS3 Coulter Counter OK 230215 £free n/a
AutoMACSPro OK 280415 £48+£30 p/h n/a
       
Advanced notice of refurbishments in E6
We are pleased to announce that new pieces of equipment will be arriving in the Hub over next 6-9 months. To optimise the space and to enable this expansion, we will have to undergo the minor space alterations and refurbishments. The refurbishments will affect the analyser room, sorting room, phenotyping area and the newly added room for imaging equipment. The timeline is not finalised, but the refurbishments shall take place in phases between end of May and August 2015. For short period of time (few days), analysers and confocal won’t be accessible at all, during other times they will be accessible but the environment will be noisy due to the works in adjacent rooms. We will update on dates giving you several weeks of notice as soon as we receive confirmation from the Estates Management. Unfortunately, some inconvenience for the users cannot be avoided, but we will do our best to minimise it.
 
Reminder
All new users of analysers and /or microscopes and all new clients of our sorting facility must have an induction with our director: Anna Petrunkina
 
RMS Residential Flow Cytometry Course

13th to 18th September 2015; York University

Download Flyer

Please register at this page

more “BuildingCyt” Modular Training Courses

Module 1 (single day)

5th June, 3rd September; Royal Cambridge Hotel

3rd September 2015; Babraham Research Campus

1st October 2015; Babraham Research Campus

Module 2 (2 days)

22nd and 23rd July 2015; Babraham Research Campus

22nd and 23rd October 2015; Babraham Research Campus

register to flow_training@babraham.ac.uk

Immunophenotyping Service Announcement
Our BRC Cell Phenotyping Hub offers Immunophenotyping services to Cambridge BRC members, including panel assessments on the basic (analysis), comprehensive (analysis and cell separation) and advanced (purification of phenotyped cells)

Packages (per sample/assessment/half-day instrument use ; surcharge applied for extra time, additional antibodies and MACS beads scaled up to the number of cells)

Basic Phenotyping Assessment £190

Comprehensive Phenotyping Assessment and Cell Separation £300

Comprehensive Phenotyping and Fluorescence Activated Cell Sorting £425

These services can be customised to individual needs such that services can be used instead of packages full details of service (updated 090415)

Immunophenotyping Service Announcement
* Fortessa base configuration change on the 6th October 2014: this is to accommodate an extra detector on the violet laser, but all new experiments will need to reset configuration (The master list of the names of the detectors). Hub staff are available to help during the change.
New Risk Assessment for cat2 conversion of analyser room updated 070513
Hub policy Latest statement of Hub policy-291112
CRI Amnis image stream use Use of this imaging cytometer available through Richard Grenfell The machine is housed in the CRI building on site at Addenbrooke’s