We are interested in aspects of the molecular and structural biology of HIV. We are seeking new ways to combat this virus and to eradicate it. We work on how RNA viruses such as HIV package their genetic material using new RNA modelling methods like SHAPE to identify important RNA structures involved in genome encapsidation, and how viruses select their own RNA from the cell so specifically and ways to inhibit this. We study HIV latency and ways in which we can reawake latent viruses on the path to trying to eradicate it. We are using novel techniques including trans splicing to approach this.
No evidence of ongoing evolution in replication competent latent HIV-1 in a patient followed up for two years. Mok HP, Norton NJ, Hirst JC, Fun A, Bandara M, Wills MR, Lever AML. Sci Rep. 2018 Feb 8;8(1):2639.
Innovations in the quantitative virus outgrowth assay and its use in clinical trials. Norton NJ, Fun A, Bandara M, Wills MR, Mok HP, Lever AML. Retrovirology. 2017 Dec 21;14(1):58.
Expression of Herpes Simplex Virus Thymidine Kinase/Ganciclovir by RNA Trans-Splicing Induces Selective Killing of HIV-Producing Cells. Ingemarsdotter CK, Poddar S, Mercier S, Patzel V, Lever AML. Mol Ther Nucleic Acids. 2017 Jun 16;7:140-154.
A highly reproducible quantitative viral outgrowth assay for the measurement of the replication-competent latent HIV-1 reservoir. Fun A, Mok HP, Wills MR, Lever AM. Sci Rep. 2017 Feb 24;7:43231
Promoter optimisation of lentiviral vectors for efficient insulin gene expression in canine mesenchymal stromal cells: potential surrogate beta cells. Gautam P, Recino A, Foale RD, Zhao J, Gan SU, Wallberg M, Calne R, Lever AM. J Gene Med. 2016 Oct;18(10):312-321.
Coordination of Genomic RNA Packaging with Viral Assembly in HIV-1. Hellmund C, Lever AM. Viruses. 2016 Jul 14;8(7
Emergence of host-adapted Salmonella Enteritidis through rapid evolution in an immunocompromised host. Klemm EJ, Gkrania-Klotsas E, Hadfield J, Forbester JL, Harris SR, Hale C, Heath JN, Wileman T, Clare S, Kane L, Goulding D, Otto TD, Kay S, Doffinger R, Cooke FJ, Carmichael A, Lever AM, Parkhill J, MacLennan CA, Kumararatne D, Dougan G, Kingsley RA. Nat Microbiol. 2016 Mar;1(3). pii: 15023.
Characterizing 3D RNA structure by single molecule FRET. Stephenson JD, Kenyon JC, Symmons MF, Lever AM. Methods. 2016 Jul 1;103:57-67.
A novel combined RNA-protein interaction analysis distinguishes HIV-1 Gag protein binding sites from structural change in the viral RNA leader. Kenyon JC, Prestwood LJ, Lever AM. Sci Rep. 2015 Oct 9;5:14369
Evidence that the endosomal sorting complex required for transport-II (ESCRT-II) is required for efficient human immunodeficiency virus-1 (HIV-1) production. Meng B, Ip NC, Prestwood LJ, Abbink TE, Lever AM. Retrovirology. 2015 Aug 14;12:72.
Identification of RNA helicases in human immunodeficiency virus 1 (HIV-1) replication - a targeted small interfering RNA library screen using pseudotyped and WT HIV-1. Williams CA, Abbink TE, Jeang KT, Lever AM. J Gen Virol. 2015 Jun;96(Pt 6):1484-9