Herpesvirus are large DNA viruses which have co-evolved with their human and animal hosts for millions and millions of years. Humans are infected with eight different herpesviruses causing a broad spectrum of disease ranging from the common cold sores to cancer. Besides encoding for >100 viral proteins they also express numerous small and large non-protein-coding RNAs to reprogramme their host cell environement for their own needs. We aim to increase our understanding of the underlying molecular mechanisms and identify new molecular targets for antiviral intervention.
Burger K, Mühl B, Kellner M, Rohrmoser M, Gruber-Eber A, Windhager L, Friedel CC, Dölken L, Eick D. 4-thiouridine inhibits rRNA synthesis and causes a nucleolar stress response. RNA Biol. 2013 Sep 4;10(10).
Rädle B, Rutkowski AJ, Ruzsics Z, Friedel CC, Koszinowski UH, Dölken L. Metabolic labeling of newly transcribed RNA for high resolution gene expression profiling of RNA synthesis, processing and decay in cell culture. J Vis Exp. 2013 Aug 8;(78).
Trilling M, Bellora N, Rutkowski AJ, de Graaf M, Dickinson P, Robertson K, Prazeres da Costa O, Ghazal P, Friedel CC, Albà MM, Dölken L. Deciphering the modulation of gene expression by type I and II interferons combining 4sU-tagging, translational arrest and in silico promoter analysis. Nucleic Acids Res. 2013 Jul 5.
Bancerek J, Poss ZC, Steinparzer I, Sedlyarov V, Pfaffenwimmer T, Mikulic I, Dölken L, Strobl B, Müller M, Taatjes DJ, Kovarik P. CDK8 kinase phosphorylates transcription factor STAT1 to selectively regulate the interferon response. Immunity. 2013 Feb 21;38(2):250-62. doi: 10.1016/j.immuni.2012.10.017.