The NIHR BRC-Cambridge Phenotyping Hub has 13 cell sorters, 7 of them housed in Class II Microbiological Safety Cabinets. Our AriaIII, AriaFusion and Influx machines have high-end specification. Our FACSJazz, FACSMelody and BioRads3 instruments are more basic. All machines are charged at £121 per hour
All new clients of our sorting facility must have a general facility induction : Hub Request Listserv
| AriaIII (x3) | Aria-Fusions (x2) | Jazz (x2) | Influx (x3) | |
|---|---|---|---|---|
| lasers/colours | 4/13 | 5/18 & 4/16 | 3/6 | 4/16 |
| safety level | cat2 inc blood | cat2 (cell lines) | cat1 | cat2 inc blood |
| nozzles | 70,85,100 | 70,85,100 | 100 | 70,86,100 |
| type | cuvette | cuvette | jet-in-air (faster) | jet-in-air (faster) |
| sort streams | 4 | 4 | 2 | 6 |
| 96 well plates | yes | yes | yes | yes |
| sample cooling | yes | yes | no | yes |
| collection cooling | yes | yes | no | yes |
| lasers | 405,488,561,633 | UV,405,488,561,633 | 405,488,561 | 405,488,561,633 |
Sorting-Related Downloads
Biosafety form (modified 17th October 2023)
Sorting request form updated December 2021
Feedback Form
Feedback Form Download
Return address for feedback forms brc-e6-hub-admin@lists.cam.ac.uk
Forward Plan for Phenotyping Hub pricing
| 2021-2022 | 2022-2023 | 2023-2024 | 2023-2024 | |
|---|---|---|---|---|
| Sorting p/h (rounded up) | 105 | 110 | 121 | 122 |
| Analysis p/h in 15m increments | 40 | 43 | 46 | 46 |
| Phenotyping (example for one assessment) | 300 | 315 | 331 | 348 |
| Operator Assistance p/h | 20 | 21 | 22 | 23 |
| Self Operated Sorting | 60 | 63 | 66 | 70 |
