The NIHR Cambridge BRC Cell Phenotyping Hub is an important part of Cambridge BRC infrastructure and, simultaneously, is a part of the cross-cutting Inflammation, Infection and Immune-therapeutics research theme. The Hub delivers advanced scientific technologies supporting the excellence in research for a partnership between the University of Cambridge and Cambridge University Hospitals NHS Foundation Trust.
The Hub’s mission is to provide state-of-the-art resources and cost-effective high quality scientific, educational and technological services across the wide range of cytomics. These services fall mainly into three major areas: research, clinical and technology services and include (but are not limited to) cell and tissue processing, cell isolation (sorting), cell analysis, cell and tissue imaging, and cell phenotyping. A further function of the Hub is to collect and to disseminate the unique technological and scientific expertise crucial for promoting and supporting patient-based basic research and for translating basic biological findings into clinical practice. The Hub drives technological innovations and applies technical and instrumental advances to expand the portfolio of services and fulfil the emerging research needs. Through our collaborative Research and Development service we foster and fortify collaborations that result in many major publications.
The Hub is equipped with state-of the art equipment including high speed cell sorters, bench top analysers, microscopes and high content/high throughput equipment.
The Hub laboratories are located on Level 6 of the Addenbrooke’s main hospital block in E6. For access to the Hub services, all new clients and prospective collaborators are invited to attend a Hub induction with our Director (apl. Professor Anna Petrunkina-Harrison) to discuss their research needs and to customise the provision of services. Project-focussed consultations, theory courses and practical tutorials on instruments can be arranged after completing the induction. For all the bookings, cancellations and reporting any technical problems please contact our urgent enquiries mailing list.
Facility Status
| Machine | Status | QC date | Charge |
|---|---|---|---|
| Fortessa analyser | OK | 021119 | £28 p/h |
| Fortessa2UV analyser | OK | 301019 | £28 p/h |
| Fortessa HTS | cleaned | 120819 | n/a |
| CantoII analyser | OK | 160919 | £28 p/h |
| FacsCalibur analyser | OK | 240719 | £28 p/h |
| Attune NxT3 | OK | 160919 | £28 p/h |
| Attune NxT4 | JCBC | £28 p/h | |
| Accuri C6 | OK | 270618 | £28 p/h |
| Accuri C6 Plus | OK | 270519 | £28 p/h |
| Cellomics XTI | OK | 260218 | £28 p/h |
| AriaIII cell sorter | OK | 200819 | £85 p/h |
| Aria-fusion | OK | 200819 | £85 p/h |
| Aria-fusion2 | JCBC | 040319 | £85 p/h |
| Influx | OK | 290819 | £85 p/h |
| Influx2 | JCBC | 060516 | £85 p/h |
| FACSJazz Cell Sorter | JCBC | n/a | £85 p/h |
| FACSJazz2 Cell Sorter | JCBC | n/a | £85 p/h |
| Melody Cell Sorter | JCBC | 040319 | £47 |
| Leica SPE confocal | OK | 280618 | £28 p/h |
| Leica Sp5 confocal | OK | 180418 | £28 p/h |
| Leica LMD6 | OK | 060516 | £28 p/h |
| Seahorse XF96 | JCBC | n/a | £35 p/h |
| Cyan | JCBC | 160919 | £28 p/h |
| BioRad S3 sorter | JCBC | 160919 | £28 p/h |
| Zeiss Axioskop ‘scope | OK | 250712 | £free |
| Cytek Analyser workshop: 19th November Dept of Pathology, Tennis Court Road | |
| The Cytek Aurora is a 5 laser 40 parameter analyser relying on novel technology. Click on this link to book a session. | |
| Fluorochrome selection guide for the Aurora. | |
| One Day FCUK meeting, 21st November 2019, Babraham Institute | |
| Non RMS Member Rate £110. Student Rate £60 late fee after 31st October, Registration closes 8th November | |
| Fluidigm Users Group Meeting, 7th to 8th November | |
| Bramall Music Building, University of Birmingham Campus, Birmingham, UK registration link | |
| Imperial College Flow Cytometry Core Manager Position | |
| Details on link |