The NIHR Cambridge BRC Cell Phenotyping Hub is an important part of Cambridge BRC infrastructure and, simultaneously, is a part of the cross-cutting Inflammation, Infection and Immune-therapeutics research theme. The Hub delivers advanced scientific technologies supporting the excellence in research for a partnership between the University of Cambridge and Cambridge University Hospitals NHS Foundation Trust.

The Hub’s mission is to provide state-of-the-art resources and cost-effective high quality scientific, educational and technological services across the wide range of cytomics. These services fall mainly into three major areas: research, clinical and technology services and include (but are not limited to) cell and tissue processing, cell isolation (sorting), cell analysis, cell and tissue imaging, and cell phenotyping. A further function of the Hub is to collect and to disseminate the unique technological and scientific expertise crucial for promoting and supporting patient-based basic research and for translating basic biological findings into clinical practice. The Hub drives technological innovations and applies technical and instrumental advances to expand the portfolio of services and fulfil the emerging research needs. Through our collaborative Research and Development service we foster and fortify collaborations that result in many major publications.

The Hub is equipped with state-of the art equipment including high speed cell sorters, bench top analysers, microscopes and high content/high throughput equipment.

The Hub laboratories are located on Level 6 of the Addenbrooke’s main hospital block in E6. For access to the Hub services, all new clients and prospective collaborators are invited to attend a Hub induction with our Director (apl. Professor Anna Petrunkina-Harrison) to discuss their research needs and to customise the provision of services. Project-focussed consultations, theory courses and practical tutorials on instruments can be arranged after completing the induction.

Mailing Lists

For all announcements and updates please subscribe to our BRC Hub User List
 
For all the bookings, cancellations and technical problems please e-mail BRC Hub Request
 

Feedback Form

Feedback Form Download
 
Return address for feedback forms brc-e6-hub-admin@lists.cam.ac.uk

Announcements

Dear Hub users, We are pleased to inform you that our E6 (Hospital) site is reopnening on Monday, 15th of June for non-COVID work. Please familiarise yourself with our updated work policies prior to any bookings (see our website). We want to reiterate that we will be operating at reduced capacity of 30-50% so longer waiting times are expected. We look forward to supporting your research in both locations. Please do not hesitate to contact us if you have any questions. Your cooperation is greatly appreciated; communication will be instrumental for increasing efficiency and reducing waiting times.

Social Distancing and Safe Working Procuedures for NIHR Cambridge BRC Cell Phenotyping Hub version 1st June 2020

Facility Status and Prices from 1st April 2021
Machine	Location	QC date	Charge
JCBC Accuri C6 Plus Kirby	JCBC L00-R19	out of action	£40 p/h
JCBC Cellomics XTI	JCBC L00-R13	260218	£40 p/h
JCBC Attune NxT4	JCBC L00-R13	190721	£40 p/h
JCBC FACSJazz: Sonic	JCBC L00-R19	260121	£105 p/h
JCBC FACSJazz:Tails	JCBC L00-R19	260121	£105 p/h
JCBC Cyan	JCBC L00-R13	out of action	£40 p/h
JCBC BioRad S3 sorter	JCBC L00-R14	out of action	£40 p/h
JCBC Influx: Link	JCBC L00-R19	290621	£105 p/h
JCBC Influx: Zelda	JCBC L00-R19	120721	£105 p/h
JCBC X20s 5 laser Luigi	JCBC L00-R14	190721	£40 p/h
JCBC X20s 4 laser Mario	CL3	200120	n/a
JCBC Fortessa Peach	JCBC L00-R14	190721	£40 p/h
JCBC LSR-II PacMan	JCBC L00-R14	no violet laser	£40 p/h
JCBC Seahorse XF96	JCBC L04 R58	n/a	£40 p/h
e6 Fortessa 1	e6	290421	£40 p/h
e6 Fortessa2UV analyser	e6	130721	£40 p/h
e6 Fortessa 1 HTS	e6	120421	n/a
e6 Fortessa 2 HTS	e6	091220	n/a
e6 CantoII analyser	e6	out of action	£40 p/h
e6 Attune NxT3	e6	191020	£40 p/h
e6 Accuri C6	e6	201020	£40 p/h
e6 Leica SPE confocal	floor 5 main lab block	230720	£40 p/h
e6 Leica Sp5 confocal	floor 5 main lab block	180920	£40 p/h
e6 Leica LMD6	e6	060520	£40 p/h
e6 Influx	OK	220121	£105 p/h
e6 Zeiss Axioskop ‘scope	e6	out of action	£free
AriaIII cell sorter	OK	280621	£105 p/h
Aria-Fusion	OK	190721	£105 p/h
Melody Cell Sorter (self operated)	OK	061019	£60
Cytek Aurora	OK		£40

Forward Plan for Phenotyping Hub pricing
	2021-2022	2022-2023	2023-2024	2023-2024
Sorting p/h (rounded up)	105	110	116	122
Analysis p/h in 15m increments	40	42	44	46
Phenotyping (example for one assessment)	300	315	331	348
Operator Assistance p/h	20	21	22	23
Self Operated Sorting	60	63	66	70