The NIHR Cambridge BRC Cell Phenotyping Hub is an important part of Cambridge BRC infrastructure and, simultaneously, is a part of the cross-cutting Inflammation, Infection and Immune-therapeutics research theme. The Hub delivers advanced scientific technologies supporting the excellence in research for a partnership between the University of Cambridge and Cambridge University Hospitals NHS Foundation Trust.
The Hub’s mission is to provide state-of-the-art resources and cost-effective high quality scientific, educational and technological services across the wide range of cytomics. These services fall mainly into three major areas: research, clinical and technology services and include (but are not limited to) cell and tissue processing, cell isolation (sorting), cell analysis, cell and tissue imaging, and cell phenotyping. A further function of the Hub is to collect and to disseminate the unique technological and scientific expertise crucial for promoting and supporting patient-based basic research and for translating basic biological findings into clinical practice. The Hub drives technological innovations and applies technical and instrumental advances to expand the portfolio of services and fulfil the emerging research needs. Through our collaborative Research and Development service we foster and fortify collaborations that result in many major publications.
The Hub is equipped with state-of the art equipment including high speed cell sorters, bench top analysers, microscopes and high content/high throughput equipment.
The Hub laboratories are located on Level 6 of the Addenbrooke’s main hospital block in E6. For access to the Hub services, all new clients and prospective collaborators are invited to attend a Hub induction with our Director (apl. Professor Anna Petrunkina-Harrison) to discuss their research needs and to customise the provision of services. Project-focussed consultations, theory courses and practical tutorials on instruments can be arranged after completing the induction.
Mailing Lists
For all announcements and updates please subscribe to our BRC Hub User List
For all the bookings, cancellations and technical problems please e-mail BRC Hub Request
Feedback Form
Feedback Form Download
Return address for feedback forms brc-e6-hub-admin@lists.cam.ac.uk
Announcements
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Dear Hub users,
We are pleased to inform you that our E6 (Hospital) site is reopnening on Monday, 15th of June for non-COVID work. Please familiarise yourself with our updated work policies prior to any bookings (see our website). We want to reiterate that we will be operating at reduced capacity of 30-50% so longer waiting times are expected. We look forward to supporting your research in both locations. Please do not hesitate to contact us if you have any questions. Your cooperation is greatly appreciated; communication will be instrumental for increasing efficiency and reducing waiting times. |
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| Social Distancing and Safe Working Procuedures for NIHR Cambridge BRC Cell Phenotyping Hub version 1st June 2020 |
Facility Status and Prices from 1st April 2021 |
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| Machine | Location | QC date | Backup | Charge |
|---|---|---|---|---|
| JCBC Accuri C6 Plus Kirby | JCBC L00-R19 | out of action | £40 p/h | |
| JCBC Cellomics XTI | JCBC L00-R13 | 260218 | £40 p/h | |
| JCBC Attune NxT4 | JCBC L00-R13 | 190721 | £40 p/h | |
| JCBC FACSJazz: Sonic | JCBC L00-R19 | 260121 | £105 p/h | |
| JCBC FACSJazz:Tails | JCBC L00-R19 | 260121 | £105 p/h | |
| JCBC Cyan | JCBC L00-R13 | out of action | £40 p/h | |
| JCBC Cytek Aurora | JCBC L00-R13 | 030921 | £40 | |
| JCBC BioRad S3 sorter | JCBC L00-R14 | out of action | £40 p/h | |
| JCBC Influx: Link | JCBC L00-R19 | 030921 | £105 p/h | |
| JCBC Influx: Zelda | JCBC L00-R19 | 090921 | £105 p/h | |
| JCBC X20s 5 laser Luigi | JCBC L00-R14 | 190721 | £40 p/h | |
| JCBC X20s 4 laser Mario | CL3 | 200120 | n/a | |
| JCBC Fortessa Peach | JCBC L00-R14 | 190721 | £40 p/h | |
| JCBC LSR-II PacMan | JCBC L00-R14 | no violet laser | Oct 2021 | £40 p/h |
| JCBC Seahorse XF96 | JCBC L04 R58 | n/a | £40 p/h | e6 Fortessa 1 | e6 | 290421 CST | £40 p/h |
| e6 Fortessa2UV analyser | e6 | 230721 CST | £40 p/h | |
| e6 Fortessa 1 HTS | e6 | 120421 | Apr 2021 | n/a |
| e6 Fortessa 2 HTS | e6 | 230721 | n/a | |
| e6 CantoII analyser | e6 | out of action | Aug 2020 | £40 p/h |
| e6 Attune NxT3 | e6 | 111021 | Oct 2020 | £40 p/h |
| e6 Accuri C6 | e6 | 200421 | £40 p/h | |
| e6 Leica SPE confocal | floor 5 main lab block | 230720 | £40 p/h | |
| e6 Leica Sp5 confocal | floor 5 main lab block | 170821 | £40 p/h | |
| e6 Leica LMD6 | e6 | 060520 | £40 p/h | |
| e6 Influx | OK | 220721 | £105 p/h | |
| e6 Zeiss Axioskop ‘scope | e6 | out of action | £free | |
| AriaIII cell sorter | OK | 280621 CST | Oct 2020 | £105 p/h |
| e6 Aria-Fusion | OK | 020921 CST | Oct 2020 | £105 p/h |
| Melody Cell Sorter (self operated) | OK | 061019 | £60 | |
Forward Plan for Phenotyping Hub pricing |
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| 2021-2022 | 2022-2023 | 2023-2024 | 2023-2024 | |
|---|---|---|---|---|
| Sorting p/h (rounded up) | 105 | 110 | 116 | 122 |
| Analysis p/h in 15m increments | 40 | 42 | 44 | 46 |
| Phenotyping (example for one assessment) | 300 | 315 | 331 | 348 |
| Operator Assistance p/h | 20 | 21 | 22 | 23 |
| Self Operated Sorting | 60 | 63 | 66 | 70 |
