The NIHR Cambridge BRC Cell Phenotyping Hub is an important part of Cambridge BRC infrastructure and, simultaneously, is a part of the cross-cutting Inflammation, Infection and Immune-therapeutics research theme. The Hub delivers advanced scientific technologies supporting the excellence in research for a partnership between the University of Cambridge and Cambridge University Hospitals NHS Foundation Trust.

The Hub’s mission is to provide state-of-the-art resources and cost-effective high quality scientific, educational and technological services across the wide range of cytomics. These services fall mainly into three major areas: research, clinical and technology services and include (but are not limited to) cell and tissue processing, cell isolation (sorting), cell analysis, cell and tissue imaging, and cell phenotyping. A further function of the Hub is to collect and to disseminate the unique technological and scientific expertise crucial for promoting and supporting patient-based basic research and for translating basic biological findings into clinical practice. The Hub drives technological innovations and applies technical and instrumental advances to expand the portfolio of services and fulfil the emerging research needs. Through our collaborative Research and Development service we foster and fortify collaborations that result in many major publications.

The Hub is equipped with state-of the art equipment including high speed cell sorters, bench top analysers, microscopes and high content/high throughput equipment.

The Hub laboratories are located on Level 6 of the Addenbrooke’s main hospital block in E6. For access to the Hub services, all new clients and prospective collaborators are invited to attend a Hub induction with our Director (apl. Professor Anna Petrunkina-Harrison) to discuss their research needs and to customise the provision of services. Project-focussed consultations, theory courses and practical tutorials on instruments can be arranged after completing the induction. For all the bookings, cancellations and reporting any technical problems please contact our urgent enquiries mailing list.

 

Announcements

Thermofisher have set up an Attune NxT Flow Cytometer Showcase day (12th February) in conjunction with the Hub. The event will take place in the JCBC. Our specialist Lynesay McKay will be presenting an instrument overview and introduction at 11:00 in the level 4 seminar room, followed by Four Live Experience Demo’s covering Immunophenotyping, Whole Blood and Rare Event Detection, Cell Cycle Analysis and a final session covering Plate Based Assays.

Facility Status
Machine	Location	QC date	Charge
JCBC Accuri C6 Plus Kirby	JCBC L00-R19	201119	£28 p/h
JCBC Cellomics XTI	JCBC L00-R13	260218	£28 p/h
JCBC Attune NxT4	JCBC L00-R13		£28 p/h
JCBC FACSJazz: Sonic	JCBC L00-R19	n/a	£85 p/h
JCBC FACSJazz:Tails	JCBC L00-R19	n/a	£85 p/h
JCBC Cyan	JCBC L00-R13	160919	£28 p/h
JCBC BioRad S3 sorter	JCBC L00-R14	160919	£28 p/h
JCBC Influx: Link	JCBC L00-R19	200120	£85 p/h
JCBC Influx: Zelda	JCBC L00-R19	200120	£85 p/h
JCBC X20s 5 laser Luigi	JCBC L00-R14	200120	£28 p/h
JCBC X20s 4 laser Mario	JCBC L00-R14	200120	£28 p/h
JCBC Fortessa Peach	JCBC L00-R14	060120	£28 p/h
JCBC Seahorse XF96	JCBC L04 R58	n/a	£35 p/h
e6 Fortessa analyser	e6	280120	£28 p/h
e6 Fortessa2UV analyser	e6	280120	£28 p/h
e6 Fortessa HTS	e6	140120	n/a
e6 CantoII analyser	e6	200120	£28 p/h
e6 FacsCalibur analyser	e6	060120	£28 p/h
e6 Attune NxT3	e6	240120	£28 p/h
e6 Accuri C6	e6	270618	£28 p/h
e6 Leica SPE confocal	e6	280618	£28 p/h
e6 Leica Sp5 confocal	e6	180418	£28 p/h
e6 Leica LMD6	e6	060516	£28 p/h
e6 Influx	OK	290819	£85 p/h
e6 Zeiss Axioskop ‘scope	e6	250712	£free
AriaIII cell sorter	OK	200819	£85 p/h
Aria-Fusion	OK	200819	£85 p/h
Melody Cell Sorter	OK	040319	£47