My laboratory studies homeostatic abnormalities in autoimmune disease. We have developed novel methodologies for quantifying the rates of production and destruction of cells and macromolecules in cell culture and in the living body, using nonradioactive isotope labelling and mass spectrometry. We are using this approach to understand the dynamics of antigen presentation and its role as a link between polymorphisms in the major histocompatibility complex (MHC) and risk of autoimmune arthritis.
- De Riva A, Deery MJ, McDonald S, Lund T, Busch R (2010) Measurement of protein synthesis using heavy water labeling and peptide mass spectrometry: Discrimination between major histocompatibility complex allotypes Anal Biochem 403(1-2): 1-12
- Busch R *, Neese RA*, Awada M, Hayes GM, Hellerstein MK (2007) Measurement of cell proliferation by heavy water labeling Nat Protoc 2:3045-3057. (* Joint first authors)
- Busch R, Rinderknecht CH, Roh S, Lee AW, Harding JJ, Burster T, Hornell TM, Mellins ED (2005) Achieving stability through editing and chaperoning: Regulation of MHC class II peptide binding and expression Immunol Rev 207:242-260